Difficulties in whole-cell path clamp recording from ARC POMC neurons

Current-clamp in whole-cell patch clamping is apparently more difficult than other types of electrophysiological recordings such as voltage-clamp because cells are less stable.

Moreover, POMC neurons in the ARC are more difficult to record from for their size (?) and other reasons.

And recordings from the ARC are difficult in general because of high movement of the tissue because it is positioned close to the third ventricle.

Finally, due to variable and low degree of co-expression of appetite-regulating peptides and serotonin receptors, the current project I'm working on takes that much longer.